Recombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2kD disulfide-linked homodimeric protein (aa207-371), consisting of two 165 amino acid polypeptides.
ED50 is < 3ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
E. coli
Form
Supplied as a lyophilized powder from 20mM HOAc. No preservatives added. Reconstitute with 4mM HCl to a concentration of 0.1-1.0mg/ml.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. VEGF serum levels are not associated with Parkinson's disease Eur. J. Neurol. 14 (8), E6 (2007). 2. Association between vascular endothelial growth factor gene polymorphisms and survival in hepatocellular carcinoma patients Hepatology 46 (2), 446-455 (2007).