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You are here:Home » Molecular Biology » Growth Factors-VEGF » Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)

Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)

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Specifications

Catalog #V2110-15H
DescriptionRecombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2kD disulfide-linked homodimeric protein (aa207-371), consisting of two 165 amino acid polypeptides.
SourceRecombinant human VEGF165 expressed in E. coli.
Amino Acide SequenceApmaegggqnhhevvkfmdvyqrsychpietlvdifqeypdeieyifkpscvplmrcggccndeglecvPteesnitmqimrikphqgqhigemsflqhnkcecrpkkdrarqenpcgpcserrkhlfvqdpqtckcsckntdsrckarqlelnertcrcdkprr
Biological ActivityED50 is < ng/ml as determined by the dose-dependent stimulation
of the proliferation of human umbilical vein endothelial cells (HUVEC).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight38.2kD
SourceE. coli
Purity98% (SDS-PAGE and HPLC) Endotoxin: 1EU/ug (LAL).
FormSupplied as a lyophilized powder from 20mM HOAc. No preservatives added. Reconstitute with 4mM HCl to a concentration of 0.1-1.0mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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