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Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)
Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)
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| Catalog # | V2110-15H | | Description | Recombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2kD disulfide-linked homodimeric protein (aa207-371), consisting of two 165 amino acid polypeptides. | | Source | Recombinant human VEGF165 expressed in E. coli. | | Amino Acide Sequence | ApmaegggqnhhevvkfmdvyqrsychpietlvdifqeypdeieyifkpscvplmrcggccndeglecvPteesnitmqimrikphqgqhigemsflqhnkcecrpkkdrarqenpcgpcserrkhlfvqdpqtckcsckntdsrckarqlelnertcrcdkprr | | Biological Activity | ED50 is < ng/ml as determined by the dose-dependent stimulation | | of the proliferation of human umbilical vein endothelial cells (HUVEC). | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 38.2kD | | Source | E. coli | | Purity | 98% (SDS-PAGE and HPLC) Endotoxin: 1EU/ug (LAL). | | Form | Supplied as a lyophilized powder from 20mM HOAc. No preservatives added. Reconstitute with 4mM HCl to a concentration of 0.1-1.0mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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