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You are here:Home » Molecular Biology » Growth Factors-VEGF » Vascular Endothelial Growth Factor, Recombinant, Human (VEGF, Vascular Endothelial Growth Factor A, VEGFA, VEGF-A, MGC70609, MVCD1, Vascular Permeability Factor, VPF)

Vascular Endothelial Growth Factor, Recombinant, Human (VEGF, Vascular Endothelial Growth
Factor A, VEGFA, VEGF-A, MGC70609, MVCD1, Vascular Permeability Factor, VPF)

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Specifications

Catalog #V2110-15T
SourceRecombinant corresponding to 165aa of human Vascular Endothelial Growth Factor expressed in E.coli is a double, non-glycosylated, polypeptide chain.
Molecular Weight~38.2kD
Biological ActivityThe biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 nug/ml.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~38.2kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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