VEGF165, Recombinant, Human, aa207-371, His-tag (Vascular Endothelial Growth Factor A
Isoform 165A, VEGF 165, VEGF165, VEGF165A, VEGFA)
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| VEGF is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. | | | Catalog # | V2110-15V | | Source | Recombinant corresponding to aa207-371 from human VEGF165, fused to His-tag at N-terminal expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC | | Molecular Weight | ~21.3kD (MALDI-TOF) | | Endotoxin | ~1EU/ug | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~21.3kD | | Source | E.coli | | Purity | ~90% (SDS-PAGE) | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.5, 50% glycerol, 5mM DTT, 200mM sodium chloride, 2mM EDTA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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