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You are here:Home » Antibodies » Abs to Receptors » Anti -VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, FLK-1, KDR) (VEGFR2, VEGFR 2, Protein-tyrosine kinase receptor flk-1, Fetal liver kinase 1, Kinase NYK, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor

Anti -VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, FLK-1, KDR)
(VEGFR2, VEGFR 2, Protein-tyrosine kinase receptor flk-1, Fetal liver kinase 1, Kinase
NYK, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase
receptor

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as fetal liver kinase 1 (FLK-1) in mouse and KDR in human, is a transmembrane tyrosine kinase that plays an important role in the development of the vascular system and the vascularization of a wide variety of tumors. Binding of VEGF to VEGFR-2 leads to tyrosine phosphorylation of the dimerized receptor and subsequent phosphorylation of SH2-containing intracellular signaling proteins, including phospholipase C, Src family tyrosine kinase and phosphatidylinositol 3-kinase (PI3K) adaptor molecules, SHC, NCK, and Ras GTPase-activating protein. VEGFR-2 is expressed in cultured human umbilical vein endothelial cells (HUVEC), Hela cells, WM 35 melanoma cells, WM 9 melanoma cells, as well as mouse NIH3T3, and Balb/c 3T3 cells.
In a single study of 115 paraffin-embedded cancer specimens analyzed by immunohistochemistry, VEGFR-2 expression was observed in bladder tumors, breast cancer, intestinal cancer and lung cancer, suggesting a direct role for VEGF as an autocrine growth factor in a variety of human cancers.
Catalog #V2110-18F
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the N-terminal region of the mouse vascular endothelial growth factor receptor-2 (VEGFR-2, FLK-1, KDR) protein.
SpecificityRrecognizes mouse VEGF Receptor-2. Identi es the ~230kD mature form of VEGFR-2. Reactivity is con rmed with mouse SVEC 410 cell lysates. Human reactivity is expected based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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