Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1175) (VEGFR2,
KDR, Flk-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
E B IH IC IF |
|
| Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLC gamma as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). | | | Catalog # | V2110-18J | | Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunohistochemistry (Paraffin): 1:300 | | Immunofluorescence (IC): 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 7H230 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. | | Purity | Supernatant. | | Immunogen | Synthetic peptide corresponding to residues surrounding Tyr1175 of human VEGF receptor 2 (KLH). MW: 230kD. | | Specificity | Recognizes endogenous levels of human VEGFR-2 at ~230kD only when phosphorylated at tyrosine 1175. Does not crossreact with other phosphorylated receptor tyrosine kinases. May crossreact with VEGFR1. Species Crossreactivity: Mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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