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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)

Anti -VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert
domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial
growth factor receptor 2, VEGFR, VEGFR2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IP IH IC FC IF
Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for GRB2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCg, as well as Shb (5, 6). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4).
Catalog #V2110-18M
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immonofluorescence, Flow Cytometry, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:300
Immunoprecipitation: 1:100
Immunofluorescence (IF-IC): 1:100
Immunofluorescence (IF-F): 1:100
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4i6
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
PuritySupernatant.
ImmunogenGST-fusion protein containing the carboxy-terminal 150aa
residues of human VEGF receptor 2.
SpecificityRecognizes endogenous levels of human VEGF receptor 2 protein at 210, 230kD. Does not crossreact with other family members. Species Crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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