Anti -VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert
domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial
growth factor receptor 2, VEGFR, VEGFR2)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
E B IP IH IC FC IF |
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| Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for GRB2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCg, as well as Shb (5, 6). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4). | | | Catalog # | V2110-18M | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immonofluorescence, Flow Cytometry, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunohistochemistry (Paraffin): 1:300 | | Immunoprecipitation: 1:100 | | Immunofluorescence (IF-IC): 1:100 | | Immunofluorescence (IF-F): 1:100 | | Flow Cytometry: 1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 4i6 | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. | | Purity | Supernatant. | | Immunogen | GST-fusion protein containing the carboxy-terminal 150aa | | residues of human VEGF receptor 2. | | Specificity | Recognizes endogenous levels of human VEGF receptor 2 protein at 210, 230kD. Does not crossreact with other family members. Species Crossreactivity: Mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2) |
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