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You are here:Home » Antibodies » Abs to Receptors » Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2,
Flk1, KDR, Receptor Tyrosine Kinase)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IH
Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2. The phosphorylation of Tyr1212 provides a docking site for Grb2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCg, as well as Shb. Signaling from VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo.
Catalog #V2110-19
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:125 Use citrate as the unmasking buffer and TBST-5%NGS as antibody diluent.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No5i133
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2 (KLH).
SpecificityRecognizes human VEGF receptor 2 only when phosphorylated at Tyr951 at ~230kD. May crossreact with activated VEGF receptor 1, but does not react with other related tyrosine phosphorylated tyrosine kinases. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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