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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054) (VEGF R2,
Flk1, KDR, Receptor Tyrosine Kinase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Vascular endothelial growth factor receptor 2 (VEGFR2, also known as KDR/FLK-1) is a 200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine bindingcontaining signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.
Catalog #V2110-19C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.35-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlNIH3T3 cells transfected with full-length, wild type VEGFR2, or Porcine Aortic Epithelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the VEGFR2.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenChemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1054.
SpecificityRecognizes mouse VEGFR2. Species sequence homology: Human and rat VEGFR3 (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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