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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054, Tyr1059) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054, Tyr1059)
(VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine binding domain containing signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.
Catalog #V2110-19D
ApplicationsSuitable for use in Western blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution to be determined by researcher.
Positive ControlsPorcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF-1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR2.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in Dulbecco PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG and Protease free), 50% glycerol , pH 7.3, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic phosphopeptide derived from the region of human VEGFR2. This sequence is conserved in mouse and rat.
SpecificityRecognizes human VEGFR2 when phosphorylated at Tyr1054 and Tyr1059. Sequence Homology (100%)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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