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You are here:Home » Antibodies » Abs to Peptides » Anti -Vasoactive Intestinal Peptide (VIP)

Anti -Vasoactive Intestinal Peptide (VIP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
Located in the nerve fibers in the central and peripheral nervous system, VIP is probably acting as a neurotransmitter. The Verner-Morrison or Water Diarrhea Hypokalemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. VIP-producing tumors are usually neuroblastomas or endocrine tumors in the pancreas. The localization of the antigen is extracellular and cytoplasmic.
Catalog #V2115-08
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen and Paraffin sections): 1:2500-1:5000 using PAP, or 1:1500-1:3000 using FITC with overnight incubation at 2-8°C.
Optimal dilutions to be determined by the researcher.
Positive ControlFormalin-fixed paraffin sections of feline ileum.
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourcePorcine
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with 50ul 40% glycerol, ddH2O. Further dilute with PBS, 1% BSA, 0.1% sodium azide.
PuritySerum
ImmunogenPurified porcine VIP
SpecificityRecognizes porcine VIP. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PHI, secretin, glucagon, GIP, and CCK do not. Species Crossreactivity: Fish and most mammals including human, feline and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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