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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP FC

Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. Three mammalian members Vav1, Vav2 and Vav3, have been identified. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the
immune synapse. Vav2 and Vav3 are more ubiquitously expressed. In addition to the Dbl homology (DH) domain, which confers GEF activity, Vavs contain protein interaction domains that allow them to function in various pathways regulating organization of the actin cytoskeleton (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
Catalog #	V2121-01
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. Other methods have not been tested.
Recommended Dilution	Western blotting 1:1000
	Immunoprecipitation 1:50
	Flow Cytometry 1:25
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Tyr174 of human Vav (KLH coupled).
Specificity	Detects endogenous levels of human Vav. Does not crossreact with Vav2, Vav3 or CelVav. Species Crossreactivity: Mouse and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.