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Abs to GTPase, Rab, Ras, Rho Proteins
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Anti -Vav2 (VAV2, vav 2 oncogene, Oncogene VAV2, Guanine nucleotide exchange factor, Gef)
Anti -Vav2 (VAV2, vav 2 oncogene, Oncogene VAV2, Guanine nucleotide exchange factor, Gef)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP |
|
| Vav2 is an oncoprotein member of the Dbl family of proteins that serves as a guanine nucleotide exchange factor (GEF) for the Rho family of Ras-related GTPases (1). Vav2 possesses a C-terminal pleckstrin homology (PH) domain and a tandem Dbl homology (DH) domain which interacts with Rho family GTPases to catalyze GDP release (1-2). The PH | | | domain serves as a negative regulator of DH domain GEF activity, which is promoted by phosphatidylinositol 4, 5-phosphate (PIP2) and is antagonized by the PI3K product, phosphatidylinositol3, 4, 5-phosphate (PIP3) (3). Hence, PI3K may facilitate the activation of Vav. Vav2 also contains a cysteine-rich domain (CRD) that is a positive modulator for DH | | | domain function (4). Vav2 is widely expressed in human tissues (5-6). The SH2 domain of Vav2 associates with the receptor tyrosine kinases EGFr (7-9) and PDGFr (8), binding preferentially to the phosphorylated residues pTyr-992 and pTyr-1148 of EGFr (10) and directly to multiple autophosphorylation sites of PDGFr (8). Overexpression of Vav2 has been shown to be sufficient to activate its oncogenic potential (6). In ovarian tumor cells, the binding of hyaluronan to its receptor (CD44v3) and the recruitment of Grb2 and p185HER2 to the CD44v3-Vav2 complex activates Vav2-mediated Rac1 and Ras signaling, leading to ovarian tumor cell migration and growth. | | | Catalog # | V2121-10 | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. | | Recommended Dilution | ELISA: 0.1-1ug/ml | | Western Blot: 1-3ug/ml | | Immunoprecipitation: 1-5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide derived from the C-terminal region of the human Vav2 protein. | | Specificity | Speci c for the human Vav2 protein. On Western blots, it identi es a single band at ~100kD. Reactivity has been con rmed with human T47D breast carcinoma, SK-OV3 ovarian carcinoma and U-937 lymphoma cell lysates. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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