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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Anti -Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef,
GDP/GTP Exchange Factor, Gef)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement. VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of VAV 3.
Catalog #V2121-15B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-2ug/ml of this lot detected mouse Vav3 in Jurkat cells transfected with murine Vav3.
Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate Vav3 from primary mouse thymocytes and overexpressed myc-tagged murine Vav3 in Jurkat cell lysate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to aa566-590 (NSVEQGPFKPPEKRTNGLRRASRQV) of mouse Vav3.
SpecificityRecognizes Vav3, Mr ~98kD. Species Crossreactivity: Mouse. Slight crossreactivity to human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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