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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Anti -Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef,
GDP/GTP Exchange Factor, Gef)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC
Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement. VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of VAV 3.
Catalog #V2121-15C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detected Vav3 in RIPA lysates from HeLa and Jurkat cells.
Immunoprecipitation: Reported to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate.
Immunocytochemistry: Reported to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells.
Immunohistochemistry: Reported to detect Vav3 in pancreatic adenocarcinoma sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3 (KLH).
SpecificityRecognizes human Vav3, Mr ~98kD. Additional non-specific bands detected in long exposures at Mr ~38-40kD. A non-specific band at Mr ~95kD was detected in Jurkat lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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