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You are here:Home » Antibodies » Abs to Infectious Disease E. coli » Anti -Verotoxin I and II (Mixed clones) (SLT-1/2, STX-1/2)

Anti -Verotoxin I and II (Mixed clones) (SLT-1/2, STX-1/2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B N
Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
Catalog #V2121-41
ApplicationsSuitable for use in ELISA, Western Blot and Toxin Neutralization. Other applications not tested.
Recommended DilutionsELISA (Capture): 20ug/ml
Western Blot: 1ug/ml
Toxin Neutralization: 5ug/ml neutralizes Vero Cell toxicity of E.coli O157:H7.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone NoMixed clones
HostMouse
SourceE. coli
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 5mM phosphate buffer, pH 7.3. No preservatives added. Reconstitute with 1ml sterile ddH20.
PurityPurified by Protein A affinity chromatography.
SpecificityRecognizes E. coli Shigatoxin 1 and 2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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