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Anti -Verotoxin I and II (Mixed clones) (SLT-1/2, STX-1/2)
Anti -Verotoxin I and II (Mixed clones) (SLT-1/2, STX-1/2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B N |
|
| Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis. | | | Catalog # | V2121-41 | | Applications | Suitable for use in ELISA, Western Blot and Toxin Neutralization. Other applications not tested. | | Recommended Dilutions | ELISA (Capture): 20ug/ml | | Western Blot: 1ug/ml | | Toxin Neutralization: 5ug/ml neutralizes Vero Cell toxicity of E.coli O157:H7. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | Mixed clones | | Host | Mouse | | Source | E. coli | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from 5mM phosphate buffer, pH 7.3. No preservatives added. Reconstitute with 1ml sterile ddH20. | | Purity | Purified by Protein A affinity chromatography. | | Specificity | Recognizes E. coli Shigatoxin 1 and 2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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