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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Vimentin (FLJ36605, VIM) (FITC)

Anti -Vimentin (FLJ36605, VIM) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
The binding region of this antibody has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alphahelical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa353 in murine vimentin (that could explain for the weak crossreaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Catalog #V2122-03C
ApplicationsSuitable for use in Western Blot, FLISA, Immunofluorescence microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin. Protease pretreatment is required prior to antibody application.
Optimal dilution determined by the researcher.
Positive ControlsCultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F310
HostMouse
SourceBovine
ConcentrationAs reported
FormSupplied as a liquid. Conjugated to FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenVimentin (purified from bovine lens).
SpecificityHighly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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