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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH

Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in a variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4).
Catalog #	V2122-14
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:500
	Immunoprecipitation: 1:15
	Immunohistochemistry: 1:100-1:200; Staining pattern is cytoplasmic
	Optimal dilutions to be determined by the researcher.
Positive Control	Sarcomas
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E634
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0.1% sodium azide, 0.05% BSA, before the addition of glycerol to 40%.
Purity	Supernatant
Immunogen	Recombinant human Vimentin protein. MW of Antigen: 57kD.
Specificity	Recognizes human Vimentin. Species Crossreactivity: Mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.