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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Vimentin

Anti -Vimentin

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in a variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4).
Catalog #V2122-14
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunoprecipitation: 1:15
Immunohistochemistry: 1:100-1:200; Staining pattern is cytoplasmic
Optimal dilutions to be determined by the researcher.
Positive ControlSarcomas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E634
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1% sodium azide, 0.05% BSA, before the addition of glycerol to 40%.
PuritySupernatant
ImmunogenRecombinant human Vimentin protein. MW of Antigen: 57kD.
SpecificityRecognizes human Vimentin. Species Crossreactivity: Mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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