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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #	V2150-51
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
	ELISA: 1:100-1:1000
	Western Blot: 1:500-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG1k
Clone No	6D833
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Vitellogenin from plasma of 17 -estradiol treated striped bass (Morone saxatilis), brown bullhead (Ameiurus nebulosus), and common carp (Cyprinus carpio).
Specificity	Recognizes vitellogenin from common carp (Cyprinus carpio). Cross-reacts with vitellogenin from fathead minnow (Pimephales promelas), zebrafish (Danio rerio), goldfish (Carassiu auratus), mullet (Mugil sp.), pinfish (Lagodon rhomboides), and sucker (Catastomidea sp.). Does not cross-react with vitellogenin from striped bass (Morone saxatilis) or brown bullhead (lctalurus nebulosus).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.