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You are here:Home » Antibodies » Abs to Fish » Anti -Vitellogenin (killifish)

Anti -Vitellogenin (killifish)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #V2150-53
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:1000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1, k
Clone No6D836
HostMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 0.15M sodium chloride, 0.1% sodium azide, 1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenVitellogenin from plasma of 17beta-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus), bowfish (Xiphias gladius) and egg yolk from swordfish.
SpecificityRecognizes vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). Cossreacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). Does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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