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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #	V2150-54D
Applications	Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution	ELISA: 1:100-1:5000
	Western Blot: 1:100-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6D838
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Vitellogenin from plasma of 17-beta estradiol induced Atlantic salmon (Salmo salar).
Specificity	Recognizes Vitellogenin from Atlantic salmon. Crossreacts with Vitellogenin from brown trout (Salmo trutta) but not with Vitellogenin from rainbow trout (Oncorhynchus mykiss), Arctic char (Salvelinus alpinus), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus) or halibut (Hippoglossus hippoglossus).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.