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You are here:Home » Antibodies » Abs to Fish » Anti -Vitellogenin (stickleback)

Anti -Vitellogenin (stickleback)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #V2150-56
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:50-1:500
Western Blot: 1:50-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG2b,k
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 12.3mM sodium azide, and 1% BSA, before tha addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenVitellogenin from whole body homogenate of 17b-estradiol treated three-spined stickleback.
SpecificityRecognizes vitellogenin from stickleback (Gasterosteus aculeatus).
Species CrossreactivityRainbow trout (Oncorhynchus mykiss), tilapia (Oreochromis niloticus), sheepshead minnow (Cyprinodon variegatus), cod (Gadus morhua), turbot (Scophthalmus maximus) and ballan wrasse (Labrus bergylta).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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