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You are here:Home » Antibodies » Abs to Fish » Anti -Vitellogenin (zebrafish)

Anti -Vitellogenin (zebrafish)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #V2150-59D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: 1:100-1:1000.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,kappa
Clone No6D844
HostMouse
SourceZebrafish
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenVitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish.
SpecificityRecognizes vitellogenin in whole body homogenate samples from zebrafish. Cross-reacts with vitellogenin from carp, fathead minnow, and roach.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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