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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #	V2150-59D
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	ELISA: 1:100-1:1000.
	Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
	Western Blot: 1:100-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,kappa
Clone No	6D844
Host	Mouse
Source	Zebrafish
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Vitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish.
Specificity	Recognizes vitellogenin in whole body homogenate samples from zebrafish. Cross-reacts with vitellogenin from carp, fathead minnow, and roach.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.