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You are here:Home » Antibodies » Abs to Fish » Anti -Vitellogenin (zebrafish)

Anti -Vitellogenin (zebrafish)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Catalog #V2150-59H
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:1000. Not recommended for coating due to presence of BSA.
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6D845
HostMouse
SourceZebrafish
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
PurityPurified by affinity chromatography.
ImmunogenVitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish (Danio rerio).
SpecificityRecognizes vitellogenin in whole body homogenate samples of zebrafish (Danio rerio). Crossreactivity: Vitellogenin from other cyprinid species, including carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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