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You are here:Home » Antibodies » Abs to Vitronectin » Anti -Vitronectin (Serum Spreading Factor, Somatomedin B, Complement S-Protein)

Anti -Vitronectin (Serum Spreading Factor, Somatomedin B, Complement S-Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin circulates as a mixture of both 75kD and 65kD forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (1).
Catalog #V2200-15D
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Inhibition. Can be used to probe vitronectin conformation. Not suitable for Western Blot (2). Other applications not tested.
Recommended DilutionsELISA: 1:16,000 (1,2,3)
Immunohistochemistry (frozen PLP-fixed sections): 1:100
Inhibition: Inhibits integrin-mediated cell adhesion to bovine vitronectin.
Optimal dilutions to be determined by the researcher.
Culture mediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F208
HostMouse
SourceBovine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenLysed bovine corneal endothelial cells and extracellular matrix.
SpecificityRecognizes bovine vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: rabbit and equine. Does not recognize human or sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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