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You are here:Home » Antibodies » Abs to Vitronectin » Anti -Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Anti -Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN,
Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin
B, Somatomedin-B, V75)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75kD and 65kD forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin.
Catalog #V2200-15E
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:80000
Western Blot: 1:200
Immunohistochemistry: 1:50 using frozen, PLP-fixed sections of bovine tissues.
Optimal dilutions to be determined by the researcher.
Culture Medium
RPMI 1640 with 10% fetal calf serum
HybridomaSp2/O myeloma cells with spleen cells from female BALB/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F209
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenLysed bovine corneal endothelial cells and extracellular matrix.
SpecificityRecognizes bovine vitronectin. Species Crossreactivity: sheep. Does not crossreact with human or equine. There is no evidence for crossreactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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