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You are here:Home » Antibodies » Abs to Vitronectin » Anti -Vitronectin (VTN, Serum-spreading factor, S-protein, V75, VN, VNT)

Anti -Vitronectin (VTN, Serum-spreading factor, S-protein, V75, VN, VNT)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E
Vitronectin (Vn), previously known as serum-spreading factor or S- protein, is a plasma and serum glycoprotein with a normal concentration ranging from 200 – 400ug/ml. It exists in both a 75kD single-chain form and a 65 + 10kD two-chain form. Vn can exist in a least two different conformational forms. The majority of Vn found in the circulation is present in the native (“closed”) form. In this form, most of the binding sites for other ligands are cryptic. The second form of Vn, the denatured (“open”, multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation. This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10kD light chain to the 65kD heavy chain of the two chain form. The liver is the primary site of Vn synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages. Vn plays an important role in a number of physiological and pathophysiological processes. It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis. Vn also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form.
Catalog #V2202
ApplicationSuitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~2mg/ml
FormSupplied in 10mM HEPES, pH 7.4, 0.15M sodium chloride and 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Vitronectin purified from plasma
SpecificityRecognizes human Vitronectin. A single precipitin arc is observed with normal plasma. No arc was observed with vitronectin depleted plasma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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