| Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis/neovascularization. |
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| Catalog # | V2700-08 |
| Applications | Suitable for use in ELISA, RIA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 1:1000. Detected vWF in human placenta lysates. |
| Indirect Immunoperoxidase Staining: 1:50 |
| Immunohistochemistry: Frozen and formalin fixed sections. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-02C: IgG, H&L (HRP) Pab Dk xMo |
| I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk xMo |
| pI | 6.55 |
| Source | Ascites |
| Control | Tonsil |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q2134 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 50% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Purified human Factor VIII complex. |
| Specificity | Recognizes human von Willebrand Factor or Factor VIII related antigen at ~310kD in both plasma and tissues. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
