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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Von Willebrand Factor (vWF, Factor VIII Related Antigen)

Anti -Von Willebrand Factor (vWF, Factor VIII Related Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC IF RIA
Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis/neovascularization.
Catalog #V2700-08
ApplicationsSuitable for use in ELISA, RIA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000. Detected vWF in human placenta lysates.
Indirect Immunoperoxidase Staining: 1:50
Immunohistochemistry: Frozen and formalin fixed sections.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-02C: IgG, H&L (HRP) Pab Dk x Mo
I1904-03C: IgG, H&L (X-Absorbed) (HRP) Pab Dk x Mo
pI 6.55
Source Ascites
Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2134
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA, 0.1% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified human Factor VIII complex (Accession# NM_000552.3).
SpecificityRecognizes human von Willebrand Factor or Factor VIII related antigen at ~310kD in both plasma and tissues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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