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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig)

Anti -VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
VSIG4 (V-set and Ig domain-containing protein 4; also Z39Ig) is a 45-50kD macrophage-specific protein that belongs to the B7 family of molecules. Its constitutive expression depresses unwarranted T cell activation. Human VSIG4 is 399 amino acids (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains a 264 aa extracellular domain (ECD) (aa 20-283) and a 95 aa cytoplasmic region. The ECD contains one V-type (aa 21-131) and one C2-type Ig-like domain (aa 143-226). Mouse VSIG4 has no C2-type domain. Over aa 20-139, human VSIG4 is 82% aa identical to mouse VSIG4. Multiple splice forms exist in human that impact the ECD and TM segments. One isoform deletes aa 67-143, while two additional isoforms substitute two aas for aa 138-233. Two new isoforms remove all or part of the TM region.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Catalog #V4880
Recommended DilutionDirect ELISA: 0.5-1ug/mL detection limit for rhVSIG4 ~ 1ng/well
Western Blot: 0.1-0.2ug/mL detection limit for rhVSIG4 ~ 1ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, recombinant human VSIG4.
SpecificityRecognizes human VSIG4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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