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Anti -VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig)
Anti -VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| VSIG4 (V-set and Ig domain-containing protein 4; also Z39Ig) is a 45-50kD macrophage-specific protein that belongs to the B7 family of molecules. Its constitutive expression depresses unwarranted T cell activation. Human VSIG4 is 399 amino acids (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains a 264 aa extracellular domain (ECD) (aa 20-283) and a 95 aa cytoplasmic region. The ECD contains one V-type (aa 21-131) and one C2-type Ig-like domain (aa 143-226). Mouse VSIG4 has no C2-type domain. Over aa 20-139, human VSIG4 is 82% aa identical to mouse VSIG4. Multiple splice forms exist in human that impact the ECD and TM segments. One isoform deletes aa 67-143, while two additional isoforms substitute two aa’s for aa 138-233. Two new isoforms remove all or part of the TM region. | | | | | | Applications: | | | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | | Catalog # | V4880 | | Recommended Dilution | Direct ELISA: 0.5-1ug/mL detection limit for rhVSIG4 ~ 1ng/well | | Western Blot: 0.1-0.2ug/mL detection limit for rhVSIG4 ~ 1ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | NS0-derived, recombinant human VSIG4. | | Specificity | Recognizes human VSIG4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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