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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface antigen 33) (Biotin)

Anti -VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface
antigen 33) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1
precursor is 407 amino acids in length. It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region. The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229). Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108.
Catalog #V4965-24
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse VSIG1 and recombinant rat VSIG1 is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, 0.1% BSA.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa23-234 extracellular domain of mouse VSIG1, expressed in NS0 cells (BAE35812). Species Sequence Homology: rat; 94%, human; 81%
SpecificityRecognizes the extracellular domain of mouse VSIG1. Species Crossreactivity: rat. Shows ~10% crossreactivity with recombinant human VSIG1 and <1% crossreactivity with recombinant mouse VSIG2, 3 and 4 and recombinant human VSIG3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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