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Specifications

5'-A T^T A A T-3'
3'-T A A T^T A-5'
Catalog #	V4990
Enzyme Properties	Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37°C.
Thermal Inactivation	Enzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNA	A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends	Csp6I, MaeI, NdeI, Tru1I
Number of Recognition Sites in DNA	Lambda: 17
	PhiX174: 2
	M13mp18/19: 7
	pBR322: 1
	pUC18/19: 3
	pUC57: 3
	pTZ19R/U: 3
	pBluescriptIIKS(-/+): 3
	pBluescriptIISK(-/+): 3
	pACYC177: 2
	pACYC184: 1
Supplied With	Provided to simply buffer selection.
	R1625: Restriction Enzyme Buffer A, 10X.
	R1625-03: Restriction Enzyme Buffer D, 10X.
Diluent Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source	Vibrio species
Concentration	10u/ul
Unit Definition	One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Quality Control
Overdigestion Assay	No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with VspI.
Ligation/Recutting Assay	After 50-fold overdigestion (3u/ug DNA x 17 hours) with VspI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.2uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay	No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.