| WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a |
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| nuclear, 36-40kD monomeric member of the WD family of repeat proteins. It is known to be |
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| expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to |
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| serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex |
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| methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone |
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| H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is |
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| 334 amino acids in length. It contains seven WD domains (aa43-333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence. |
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| Catalog # | W1006-12 |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Recombinant human WDR5, aa1-120. |
| Specificity | Recognizes endogenous human WDR5 at ~40kD. Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
