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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IF

West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.
Catalog #	W1018-50L
Applications	Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:10-1:50
	ELISA: 1:20-1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10D32
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No Stabilizing proteins added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	West Nile Virus
Specificity	Recognizes the envelope protein. Has positive reactivity with WNV and SLE flaviviruses. Has a negative reaction with JEV, YFV, DEN, TBE, EEE, WEE, POW, LAC, VEE.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.