| The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease. |
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| Catalog # | W1018-54 |
| Hybridoma | Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant West Nile Virus NS3 Protease expressed as a fusion with the cofactor NS2b, (rwnvNS3, aa 1-184 of NS3, Accession # NP_776018, and aa 49-96 of NS2b, Accession # NP_776017). |
| Applications | Western Blot, Direct ELISA |
| Recommended Dilution | Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. Using a colorimetric detection system, the detection limit for rwnvNS3 protease is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. |
| Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. The detection limit for rwnvNS3 is approximately 2 ng/well. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8H154 |
| Host | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | E.coli-derived rwnvNS3 |
| Specificity | Recognizes west nile virus NS3 protease. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
