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You are here:Home » Antibodies » Antibodies-Infectious Disease WNV » Anti -West Nile Virus, NS3 Protease (WNV)

Anti -West Nile Virus, NS3 Protease (WNV)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease.
Catalog #W1018-54
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No8H154
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding aa1-184 of NS3 (NP_776018) and aa49-96 of NS2b (NP_776017) of West Nile Virus NS3 Protease, expressed in E. coli.
SpecificityRecognizes West Nile Virus NS3 Protease.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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