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You are here:Home » Culture Media » Media-Microbiological » Wilkins Chalgren Agar (Powder)

Wilkins Chalgren Agar (Powder)


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Highly nutritive medium for cultivation and isolation of aerobic and anaerobic bacteria.
Catalog #W1050
AppearanceLight beige homogenous, free fowing powder
SolubilityYellow-beige, trace to slight hazy
pH (25ºC)7.1 ± 0.2
Formulation (g/L)Enzymatic Digest of Casein 10g
Enzymatic Digest of Gelatin 10g
Yeast Extract 5g
Sodium Chloride 5g
Dextrose 1g L-Arginine 1g
Sodium Pyruvate 1g
Hemin 0.005g
Vitamin K 0.0005g
Agar 15g
Total 47.0055
Directions per LiterDissolve 47.0055g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.
Storage and StabilityStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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