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You are here:Home » Culture Media » Media-Microbiological » Wilkins-Chalgren Broth (Powder)

Wilkins-Chalgren Broth (Powder)

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Specifications

Highly nutritive medium for cultivation and isolation of aerobic and anaerobic bacteria.
Catalog #W1050-05
AppearanceLight beige homogenous, free fowing powder
SolubilityAmber, clear
pH7.1 ± 0.2
Formulation (g/L)Enzymatic Digest of Casein 10.0
Enzymatic Digest of Gelatin 10.0
Yeast Extract 5.0
Sodium Chloride 5.0
Dextrose 1.0
L-Arginine 1.0
Sodium Pyruvate 1.0
Hemin 0.0050
Vitamin K 0.0005
Total 33.0
Directions per LiterDissolve 33g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave ((15psi) for 15 minutes at 121ºC.
Storage and StabilityStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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