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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt2B (Wingless-type MMTV (mouse mammary tumor virus) Integration Site Family, member 2B)

Anti -Wnt2B (Wingless-type MMTV (mouse mammary tumor virus) Integration Site Family,
member 2B)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis in adults1-3. Wnts are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity (also called ‘planar polarity’)2, 4,5 pathways.
Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. The Wnt/b-catenin pathway primarily regulates cell fate determination during development, whereas the main function of the Wnt polarity pathway is the regulation of cytoskeletal organization. Wnt2B/Wnt13 is expressed in the embryonic mesoderm during gastrulation, and in the dorsal midline of the diencephalons and mesencephalon, the heart primordial, the lung bud periphery, and in the otic and optic vesicles at later stages.6 Wnt2B is expressed in organs regulated by epithelial-mesenchymal interactions in the mouse embryo, such as the developing kidney, lung, salivary gland, gut, pancreas, adrenal gland, genital tubercle, eye and ear.7 Wnt2B signaling induces ureter branching during early kidney organogenesis.7 Wnt2B exists as 2 alternatively spliced isoforms (Wnt2B1 and Wnt2B2).8 Wnt2B2 is preferentially expressed in NT2 cells, with the potential of neuronal differentiation.9 In some cases of gastric cancer, Wnt2B2 up-regulation may lead to carcinogenesis through the activation of the b-catenin/TCF signaling pathway.9 Wnt2B is expressed in the immature ovary, where the Wnt signaling cascade may be involved in follicular development and ovarian function.10
Catalog #W3007
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blotting: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityEpitope-affinity purified from rabbit antiserum.
ImmunogenSynthetic peptide derived from an internal region of the human Wnt2B/Wnt13 protein.
SpecificityReacts with the human, mouse and rat Wnt2B/Wnt13 proteins. It recognizes both human Wnt13A and Wnt13B isoforms. On Western blots, it identifies a band at ~41kD. Reactivity has been confirmed with mouse Wnt2B/Wnt13-pCMV-tag2-transfected 293T, human Wnt13A- and Wnt13B-pCR3-transfected HEK293 and SK-OV-3 cell lysates, and rat ovary homogenates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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