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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family
Member)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
The Wnt family includes several secreted glycoproteins that play important roles in animal development. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. The canonical Wnt/ beta-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family. Aberrant activation of Wnt signaling pathways is involved in several types of cancers.
Catalog #W3011-02
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No8A42
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a.
SpecificityRecognizes transfected Wnt3a protein in L cells and the conditioned medium from mouse L/Wnt3a cells at ~42kD. Species Crossreactivity: mouse. Species Sequence Homology human and rat, 100%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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