Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family
Member)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The Wnt gene family consists of structurally related genes, which encode secreted signaling proteins.1 Wnt proteins have been implicated in oncogenesis 2-3 and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the Wnt-beta-catenin-TCF signaling pathway.3
Catalog #W3011-03
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-2ug/ml detects hWnt-3a by indirect ELISA using 100ul/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hWnt-3a.
Western Blot 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWnt-3a is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Wnt3 transfected 293 cells
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in sterile water to a concentration of 0.1-1mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant Wnt3a
SpecificityRecognizes human Wnt3a protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links