| Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis. |
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| Catalog # | W3021A |
| Applications | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. |
| Western Blot: 5ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 9H192 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa37-76 from mouse Wnt-4, expressed in E. coli (NP_033549). |
| Specificity | Recognizes mouse Wnt-4. Species crossreactivity: human. Does not cross-react with recombinant mouse Wnt-1, 2b, 5a, 5b, 8a, 8b, 9b, 10a, 10b, 11, 16, or with recombinant human Wnt-2, 3a, 7a, or 7b. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
