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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)

Anti -Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH
Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity.
Catalog #W3028-01
ApplicationsSuitable for use in Immunohistochemistry, ELISA, and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 25ug/ml
Western Blot: 2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11C65
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, CHO-derived, recombinant mouse Wingless related integration site family member 5a (rmWnt-5a; aa1-379; Accession # P22725).
SpecificityRecognizes mouse Wnt-5. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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