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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)

Anti -Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH
Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity.
Catalog #W3028-01
ApplicationsSuitable for use in Immunohistochemistry, ELISA, and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 8-25ug/ml
Western Blot: 10ug/ml. Use non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No11C65
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified from culture supernatant by Protein A for G affinity chromatography.
ImmunogenPurified, CHO-derived, recombinant mouse Wnt-5a corresponding to aa38-380, UniProt Accession #P22725.
SpecificityRecognizes mouse Wnt-5. Species Crossreactivity: human. By direct ELISA, no cross-reactivity observed with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11 and 16.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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