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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH

Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity.
Catalog #	W3028-01D
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000
	Western Blot: 1:500-1:2000
	Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:400
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3D10
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid, 0.03% sodium azide.
Purity	Ascites
Immunogen	Recombinant corresponding to human fragment of WNT5A.
Specificity	Recognizes human WNT5A.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.