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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt5A (Protein Wnt-5a)

Anti -Wnt5A (Protein Wnt-5a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IH
Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity.
Catalog #W3028-01D
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3D10
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.03% sodium azide.
PurityAscites
ImmunogenRecombinant corresponding to human fragment of WNT5A.
SpecificityRecognizes human WNT5A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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