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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

Anti -WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC IF
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Catalog #W3035-55B
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in human brain lysates on ~38kD bands
Immunohistochemistry (Paraffin): 3-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to CGRGHNTRTEKRKEK, from WNT3, at the internal region of the protein (NP_110380.1).
SpecificityRecognizes human WNT3. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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