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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt5b, ID (Protein Wnt-5b, wnt5b, wnt-5, wnt5, wnt5a, si:ch73-211c7.1)

Anti -Wnt5b, ID (Protein Wnt-5b, wnt5b, wnt-5, wnt5, wnt5a, si:ch73-211c7.1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
WNT5B is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt proteins constitute a large family of secreted molecules that are involved in intercellular signalling during development and have been implicated in oncogenesis. Very little is known about the structure of Wnts as they are notoriously insoluble; but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines. The Wnt proteins seem to adhere to the plasma membrane of the secreting cells and are therefore likely to signal over only few cell diameters. Fifteen major Wnt gene families have been identified in vertebrates, with multiple subtypes within some classes.
Catalog #W3035-93
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.
Control Peptide W3035-93P
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceZebrafish
ConcentrationNot determined
FormSupplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the intermediate region of zebrafish Wnt-5b.
SpecificityRecognizes zebrafish Wnt-5b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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