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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)

Anti -Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member
6 (Wnt6)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Human Wnt-6 is a 46 kD, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse and canine Wnt-6 are identical.
Catalog #W3036-01
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml
Immunohistochemistry: 5-15ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by human Wnt-6 affinity chromatography.
ImmunogenE. coli-derived recombinant human Wnt-6 peptide containing rhWnt-6 (aa35-75 and 238-299) joined by a linker peptide, Accession # Q9Y6F9
SpecificityRecognizes human Wnt-6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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