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Anti -Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)
Anti -Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IH |
|
| Human Wnt-6 is a 46 kD, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse and canine Wnt-6 are identical. | | | Catalog # | W3036-01 | | Applications | Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml | | Immunohistochemistry: 5-15ug/ml | | ELISA: 0.5-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol. | | Purity | Purified by human Wnt-6 affinity chromatography. | | Immunogen | E. coli-derived recombinant human Wnt-6 peptide containing rhWnt-6 (aa35-75 and 238-299) joined by a linker peptide, Accession # Q9Y6F9 | | Specificity | Recognizes human Wnt-6. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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