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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Wnt gene family members, including Wnt-1 through Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-6 is involved in oncogenesis and is co-expressed with Wnt10 in colorectal cancer cell lines. The presence of cervical and breast cell carcinomas may indicate an over expression of Wnt-6.
Catalog #	W3036-02
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Immunohistochemistry: 10-20ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa276 from mouse Wnt-6.
Specificity	Recognizes mouse Wnt-6 at ~40-46kD. Species Crossreactivity: human and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.