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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development.
Catalog #W3037-10
ApplicationsSuitable for use in Immunohistochemistry and Direct ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H100
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-52 and aa195-169, connected by a Gly-Ser linker from mouse Wnt-8b, expressed in E. coli (AAD31816). Species Sequence Homology: rat 100%, canine 98% and human 97%.
SpecificityRecognizes mouse Wnt-8b in direct ELISAs. Does not crossreact with recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b, -10b, rhWnt-2, -7a, -7b, or -9a. Immunohistochemical staining of human neural cell line (NTera2), demonstrates that this antibody recognizes human Wnt-8b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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