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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development.
Catalog #W3037-10
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, and ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Western Blot: 1-2ug/ml with the appropriate secondary reagents detects human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H100
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant mouse Wnt-8b
SpecificityRecognizes mouse Wnt-8b in direct ELISAs and Western Blots. Western Blots with human cell extracts demonstrate that this antibody also detects human Wnt-8b Does not crossreact with rmWnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b, -10b, rhWnt-2, -7a, -7b, or -9a. Species sequence homology: rat 100%, canine 98% and human 97%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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