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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family
Member)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development.
Catalog #W3037-10
ApplicationsSuitable for use in Immunohistochemistry and Direct ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H100
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-52 and aa195-169, connected by a Gly-Ser linker from mouse Wnt-8b, expressed in E. coli (AAD31816). Species Sequence Homology: rat 100%, canine 98% and human 97%.
SpecificityRecognizes mouse Wnt-8b in direct ELISAs. Does not crossreact with recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b, -10b, rhWnt-2, -7a, -7b, or -9a. Immunohistochemical staining of human neural cell line (NTera2), demonstrates that this antibody recognizes human Wnt-8b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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