| Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development. |
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| Catalog # | W3037-10 |
| Applications | Suitable for use in Immunohistochemistry, Western Blot, and ELISA. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4°C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. |
| Western Blot: 1-2ug/ml with the appropriate secondary reagents detects human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. |
| Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 7H100 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | E. coli-derived recombinant mouse Wnt-8b |
| Specificity | Recognizes mouse Wnt-8b in direct ELISAs and Western Blots. Western Blots with human cell extracts demonstrate that this antibody also detects human Wnt-8b Does not crossreact with rmWnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b, -10b, rhWnt-2, -7a, -7b, or -9a. Species sequence homology: rat 100%, canine 98% and human 97%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
