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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family
Member)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Mouse Wnt-8b (Wingless related integration site family member 8b) is a 40-50 kD secreted glycoprotein member of the Wnt family of proteins that function as ligands for the Frizzled family of receptors. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.
Catalog #W3037-11
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with a purified, E.coli-derived, recombinant protein consisting of mouse Wnt-8b amino acid (aa) segments 22-52 and 195-269 (Accession # AAB31816) fused via a gly/ser linker (rmWnt-8b).
ApplicationsWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk
0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween® 20
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H99
HostRat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived rmWnt-8b
SpecificitySelected for its ability to detect rmWnt-8b in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhWnt-2, -7a, -7b, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, or -9b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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