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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt9a (Wingless-type MMTV (mouse mammary tumor virus) integration site family member 9a)

Anti -Wnt9a (Wingless-type MMTV (mouse mammary tumor virus) integration site family member
9a)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic and breast cancer cells. Human Wnt-9a is 98% aa identical with both mouse and rat Wnt-9a.
Catalog #W3037-59
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml detection limit for rhWnt-9a is ~ 3ng/well.
Western Blot: 1-2ug/ml detection limit for rhWnt-9a is ~ 25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H145
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived rhWnt-9a. (aa 48 - 92 and 231 - 304)
SpecificityRecognizes human Wnt-9a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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