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Anti -Wnt9a (Wingless-type MMTV (mouse mammary tumor virus) integration site family member 9a)
Anti -Wnt9a (Wingless-type MMTV (mouse mammary tumor virus) integration site family member
9a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B |
|
| Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic and breast cancer cells. Human Wnt-9a is 98% aa identical with both mouse and rat Wnt-9a. | | | Catalog # | W3037-59 | | Applications | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1.0ug/ml detection limit for rhWnt-9a is ~ 3ng/well. | | Western Blot: 1-2ug/ml detection limit for rhWnt-9a is ~ 25ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8H145 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | E. coli-derived rhWnt-9a. (aa 48 - 92 and 231 - 304) | | Specificity | Recognizes human Wnt-9a. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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