| Mouse Wnt9b is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. The 359aa mouse Wnt9b precursor contains a 336aa mature region with 24 conserved cysteines and one potential N-linked glycosylation site. Mature mouse Wnt9b shows 93% and 98% aa identity with mature human and rat Wnt9b, respectively, and 63% aa identity with Wnt9a. Wnt9b is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver. |
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| Catalog # | W3037-60 |
| Applications | Suitable for use in Western Blot and ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-5ug/ml. Using a colormetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Used at 5ug/ml to detect Wnt9b in cell extracts. |
| Direct ELISA: 0.5-1ug/ml. The detection limit is ~100ng/well. |
| Optimal dilutions to be determined by the researcher. |
| Western Blot Buffers | Wash Buffer: 25mM Tris, pH 7.4, 0.5M sodium chloride |
| 0.05% Tween-20 |
| Blocking Buffer: 5% nonfat dry milk in wash buffer |
| Blotting Buffer: 2% nonfat dry milk in wash buffer |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 7H230 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa46-90 and 228-298 connected by a gly-ser linker from mouse Wnt9b expressed in E. coli (Accession #NP_035849). |
| Specificity | Recognizes mouse Wnt9b. Does not cross react with recombinant human Wnt2, -6, -7a, -7b, -9a, recombinant mouse Wnt1, -2b, -4, -5a, -5b, -8a, -8b, -10a, -10b, -11 or -16. |
| Species Crossreactivity | Human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
