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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt9b (Wnt-14b, Wnt-15, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Anti -Wnt9b (Wnt-14b, Wnt-15, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration
Site Family Member)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Mouse Wnt9b is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. The 359aa mouse Wnt9b precursor contains a 336aa mature region with 24 conserved cysteines and one potential N-linked glycosylation site. Mature mouse Wnt9b shows 93% and 98% aa identity with mature human and rat Wnt9b, respectively, and 63% aa identity with Wnt9a. Wnt9b is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver.
Catalog #W3037-60
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml. Using a colormetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Used at 5ug/ml to detect Wnt9b in cell extracts.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~100ng/well.
Optimal dilutions to be determined by the researcher.
Western Blot BuffersWash Buffer: 25mM Tris, pH 7.4, 0.5M sodium chloride
0.05% Tween-20
Blocking Buffer: 5% nonfat dry milk in wash buffer
Blotting Buffer: 2% nonfat dry milk in wash buffer
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H230
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa46-90 and 228-298 connected by a gly-ser linker from mouse Wnt9b expressed in E. coli (Accession #NP_035849).
SpecificityRecognizes mouse Wnt9b. Does not cross react with recombinant human Wnt2, -6, -7a, -7b, -9a, recombinant mouse Wnt1, -2b, -4, -5a, -5b, -8a, -8b, -10a, -10b, -11 or -16.
Species CrossreactivityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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