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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified B IH

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt-11 shares 96% identity over the sequences expressed in the immunogen.
Catalog #	W3038-11W
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml
	Immunohistochemistry: 8-25ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	12K195
Host	Rat
Source	Mouse
Concentration	~0.5mg/ml after reconstitution
Form	Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Recombinant corresponding to aa39-79, aa225-297 from mouse Wnt-11 expressed in E.coli.
Specificity	Recognizes mouse Wnt-11. Does not react to recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or -9a.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.