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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Wnt11 (Protein Wnt-11)

Anti -Wnt11 (Protein Wnt-11)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH
Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt-11 shares 96% identity over the sequences expressed in the immunogen.
Catalog #W3038-11W
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No12K195
HostRat
SourceMouse
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa39-79, aa225-297 from mouse Wnt-11 expressed in E.coli.
SpecificityRecognizes mouse Wnt-11. Does not react to recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or -9a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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